Figure 10.
Strategies for downstream pathogen identification and antibiotic susceptibility profiling. A. Basic bacterial differentiation principle proposed by Sun et al. The distance (d) traveled in the tapered microchannel is inversely proportional to cell stiffness. Force is drive by fluid pressure, p105. Republished with permission of Royal Society of Chemistry from, A microfluidic platform for profiling biomechanical properties of bacteria, Sun et al., 14, 2014; permission conveyed through Copyright Clearance Center, Inc. B. Plug-based microfluidic platform proposed by Boedicker et al11. Schematic of channel shows formation of nanoliter droplets containing bacteria, drug condition, and viability indicator. Republished with permission of Royal Society of Chemistry from, Detecting bacteria and determining their susceptibility to antibiotics by stochastic confinement in nanoliter droplets using plug-based microfluidics, Boedicker et al., 8, 2008; permission conveyed through Copyright Clearance Center, Inc.