Fig. 1.

CM double staining. a An 11-cc staining solution is prepared consisting of 10 cc of crystal violet and 1 cc of methylene blue. b In a separate 10-cc syringe, 1 cc of the solution is aspirated with 9 cc of air and is used for spraying.

CM double staining. a An 11-cc staining solution is prepared consisting of 10 cc of crystal violet and 1 cc of methylene blue. b In a separate 10-cc syringe, 1 cc of the solution is aspirated with 9 cc of air and is used for spraying.