Table 4.
Immunohistochemical findings
| Number Positive | N performed | % | |
|---|---|---|---|
| PAX8 | 3 | 3 | 100% |
| GATA3 | 4 | 4 | 100% |
| TTF-1 | 1 | 3 | 33% |
| CD10 | 2 | 5 | 40% |
| Calretinin | 3 | 6 | 50% |
| ER | 1 | 7 | 14% |
| PR | 0 | 5 | 0% |
| HNF-1b | 2 | 5 | 40% |
| AE1/AE3 | 2 | 2 | 100% |
| CK7 | 2 | 3 | 67% |
| EMA | 2 | 2 | 100% |
| WT-1 | 0 | 3 | 0% |
| p53 (overexpressed) | 0* | 4 | 0% |
| p16 (diffuse overexpression) | 0 | 3 | 0% |
| Mismatch Repair Proteins (retained) | 2 | 2 | 100% |
| PTEN (retained) | 2 | 2 | 100% |
| ARID1A (retained) | 2 | 2 | 100% |
| S100 | 1 | 1 | 100% |
| A103 | 0 | 1 | 0% |
| SOX10 | 0 | 1 | 0% |
| AR | 0 | 1 | 0% |
| FOXL2 | 1 | 4 | 25% |
| Inhibin | 1 | 2 | 50% |
| SF1 | 0 | 1 | 0% |
| SALL4 | 0 | 1 | 0% |
| Glypican | 0 | 1 | 0% |
| AFP | 1 | 1 | 100% |
| Thyroglobulin | 0 | 1 | 0% |
| Ki-67 proliferation index | Range 20–50% in 3 cases (1, 3 and 6) | ||
*
Case 1 - immunochemistry for P53 on the corresponding histologic specimen showed heterogenous expression (wild-type pattern). However, a subsequent recurrence one year later showed high-grade carcinoma which showed aberrant P53 overexpression. Review of the earlier histologic specimen at this time showed small foci of high-grade carcinoma morphologically similar to the recurrence.