KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| NAMPT polyclonal antibody | Bethyl | A300-372A |
| NAMPT monoclonal antibody | Adipogen | OMNI379 |
| GAPDH | Millipore | MAB374 |
| CD63 | Santa Cruz | sc-5275 |
| Alix | Santa Cruz | Sc-53540 |
| CD81 | Santa Cruz | Sc-166029 |
| CD9 | BD Pharmingen | 553758 |
| TSG101 | Abcam | ab125011 |
| Albumin | Abcam | Ab137885 |
| Transferrin | Abcam | ab82411 |
| Adiponectin | Abcam | Ab22554 |
| Mouse Complement Factor D/Adipsin | R&D systems | MAB5430 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Dextrose | Hospira, Inc | Cat# 0409-6648-02 |
| Insulin | Eli Lilly | NDC 0002-8215-01 |
| Proteinase K | Sigma | Cat# P2308 |
| Poly-L-lysine | Sigma-Aldrich | Cat# P9155 |
| AraC | Sigma | Cat# C6645 |
| DNaseI | Sigma | Cat# DN25 |
| Opti-MEM | GIBCO | Cat# 31985 |
| BODIPY TR ceramide | Thermo Fisher | Cat# D7540 |
| shNAMPT lentivirus particle | Sigma | TRCN0000101276 |
| Critical Commercial Assays | ||
| RNeasy Mini Kit | QIAGEN | Cat# 74104 |
| High-Capacity cDNA Reverse Transcription Kit | Thermo Fisher | Cat# 4368814 |
| Total Exosome Isolation Kit (from plasma) | Thermo Fisher | Cat# 4484450 |
| Exosome Spin Column | Thermo Fisher | Cat# 4484449 |
| His-3xFlag-NAMPT | N/A | |
| Experimental Models: Cell Lines | ||
| Cell line: HEK293 | ATCC | CRL-3216 |
| Cell line: OP9 | ATCC | CRL-2749 |
| Experimental Models: Organisms/Strains | ||
| Mouse: C57BL/6J | The Jackson Laboratory | RRID: IMSR_JAX:000664 |
| Mouse: Aged C57BL/6J | NIA aging colony | |
| Mouse: ANKI | N/A | |
| Software and Algorithms | ||
| Prism 5 | GraphPad | www.graphpad.com |