Fig. (11).

Wnt5a overexpression in UACC 1273 melanoma cells is associated with differential Wnt/Iý-catenin-regulated gene expression. ƒ?"(a) UACC 1273(4-7) melanoma cells which stably express Wnt5a versus UACC 1273(EV) empty vector control cells were evaluated by real-time PCR for mRNA expression of Wnt5a, survivin, cyclin D1, hnkd, and fra-1. In UACC 1273(4-7) cells which demonstrated a ~13-fold increase in Wnt5a expression, mRNA levels for survivin, cyclin D1, and hnkd all decreased significantly while levels for fra-1 message increased significantly. n ≥ 2, *p < 0.05, **p < 0.01. (b) Treatment of UACC 1273(4-7) cells with PKC inhibitor (either 3 uM Go6976 or 5 uM Ro31-8425) demonstrated suppression of mRNA expression of fra-1 and MITF as assessed by real-time PCR. n ≥ 2, *p < 0.05.