Fig. (8).

Activation of PKC enhances the interaction of Iý-catenin with p300. After respective treatments (as described below), nuclear lysates of wild type 3T3 cells were prepared and subjected to immunoprecipitation with CBP, p300, or control antibody, followed by immunoblot for Iý-catenin. (a) Treatment of 3T3 cells with specific activator of PKC PMA (100 I1/4M) for 1 h led to a modest, yet highly coactivator selective increase in Iý-catenin associated with p300. (b) 3T3 cells were treated with Wnt3a and 5a conditioned media in the presence (20 I1/4M) or absence of N-myristoylated PKC pseudosubstrate peptide specific PKCIñ inhibitor PKCIñ(20-28) for 24 h. PKCIñ(20-28) selectively inhibited the coimmunoprecipitation of Iý-catenin with p300.