Figure 3.

The level of matrix metallopeptidase 2 (MMP2) in aliquots of serum and synovial fluid from Patient 1 (index case) was analyzed using zymography. The level of MMP2 was very low in synovial fluid and was completely nonmeasurable in the serum. Normal human serum with abundant MMP2 activity was used as a control (serum aliquots of 0.5, 1, and 2 μl were used as indicated by the triangle; two different serum samples for the MONA index case were analyzed as indicated). Conditioned medium from phorphol ester treated (PMA; 4 nm) HT‐1080 fibrosarcoma cells served as a control for MMP9 and MMP2 (72 kDa pro‐form and 62 kDa active enzyme were separated by protein migration in electrophoresis prior to in‐gel activation and gelatinolysis (Lohi et al., 1996))