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. 2019 Aug 8;9:11540. doi: 10.1038/s41598-019-48041-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of Si and 3C-SiC on mitochondrial membrane potential and cell membrane integrity of OECs. (A) Representative results were taken from randomly selected slides scanned by a Nikon Ti Eclipse inverted microscope. MitoHealth (red) stains stable mitochondrial membrane potential, DEAD Green (green) stains deadly cells, Hoechst 33342 (blue) stains nuclei. A merged image for all experimental conditions was created with NIS Elements software (scale bar 50 μm). (B) Mean Fluorescence Intensities (MFI) ratio of MitoHealth (red) and DEAD Green (green) per cell. Differences in MFIs were not statistically significant between glass-plated control cells and those plated on 3C-SiC for 48 hours (3C-SiC vs Si, P < 0.005; control group vs Si, P < 0.05; differences in fluorescence intensity were not significant statistically between 3C-SiC and control group).