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. 2019 Aug 8;10:3578. doi: 10.1038/s41467-019-11452-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Scoring intratumoral and stromal myeloid lineage cells in Inflamed A and B. The IHC for case 1 at low (a) and high power magnification (b) highlights minimal CD33 + stromal neutrophils (in red) with a score of 1+ while background CD8+ stromal lymphocytes are highlighted in brown. The IHC for case 2 at low (c) and high power magnification (d) highlights a moderate CD33+ neutrophil population within the stroma with a score of 2 + with rare intratumoral CD33+ neutrophils. The IHC for case 3 at low (e) and high power magnification (f) illustrates the marked CD33+ neutrophil population within the stroma with a score of 3+. Scoring legend: 0 = virtual absence, 1 = low (<25%), 2 = moderate (25–50%), and 3 = marked increase (>50%)