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. 2019 Aug 8;9:11542. doi: 10.1038/s41598-019-47708-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Time to progression for patients with or without two occurrences of temporary increasing ctDNA levels. Patients with multiple occurrences of temporary ctDNA increases have a shorter PFS and OS than patients with stable/decreasing ctDNA levels. For one patient, only C1₀ and C4₁₄ samples were accessible and therefore this patient was excluded from this analysis. (A) Representative patient with one temporary ctDNA increase during the first 60 days of treatment. (B) Representative patient with two instances of temporary ctDNA increases. (C) Progression free survival (PFS). Boxed numbers represents the number of patients in each group not having clinical progression at the given timepoint. (D) Overall survival (OS). Boxed numbers represents the number of patients in each group alive at the indicated timepoint.