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. 2019 Aug 8;10(8):596. doi: 10.1038/s41419-019-1836-2

Fig. 2. U251 tumor spheres exhibit stemness and folate cycle signatures.

Fig. 2

a Hierarchical clustering of 100 genes involved in neural stemness reveals that 72 genes are over-expressed (yellow) and 28 genes are under-expressed (blue) in TS, n = 3; b folate signature is characterized by reduced transcription of cytosolic folate cycle genes in TS, including SHMT1, MTHFD1, and DHFR, and elevated transcription of MTHFD2L implicated in mitochondrial folate cycle, n = 3. c RT-qPCR data, n = 3, confirm the elevated expression of mitochondrial folate cycle enzyme MTHFD2L and ALDH1L2 204, an alternative transcript of ALDH1L2, in TS comparatively to ML cells; However, the expressions of SHMT1, MTHFD1, and SHMT2 do not vary significantly. *p < 0.05, **p < 0.01, ***p < 0.001, TS = tumor spheres, ML = monolayer cells,