Fig. 4.

Single-cell sequencing on the TCRα/TCRβ gene pairing and gene profile. The blister cells of a patient with CBZ-SJS/TEN (case 4) were sorted by flow cytometry using mAb recognizing human TCR TRBV12-3/TRBV12-4 (Beckman Coulter). A total of 30 lymphocytes were analyzed by single-cell sequencing to determine the V/J usage, CDR3 TCRα/TCRβ clonotypes, and the transcripts of 18 genes. a, b The specific TCRβ CDR3 clonotype “ASSLAGELF” was detected in all (100%, 30/30) single-cell clones. Twenty-five (83.33%) clones expressed the TCRα CDR3 clonotype “VFDNTDKLI”, four (13.33) clones expressed TCRα CDR3 “AASPPDGNQFY”, and one (3.33%) clone expressed TCRα CDR3 “ALDIPNFGNEKLT”. c A heatmap displays the expression values of 18 genes related to the function and differentiation of T lymphocytes in 30 single-cell clones. The corresponding TCRα and TCRβ CDR3 clonotypes are indicated to each clone