Fig. 5.

Binding response of the public TCR to different drugs and structural analogs. Surface plasmon resonance (SPR) was applied for analyzing the binding response between the soluble single-chain αβTCR (scTCR) recombinant protein and drug compounds. The scTCR composed of the TCRα CDR3 “VFDNTDKLI” and TCRβ CDR3 “ASSLAGELF” was purified from the cultured medium of HEK293F transfectants. The scTCR protein was coated to the chip, and different drugs or metabolites flowed through the chip. The binding response of the scTCR protein toward CBZ and related compounds was examined by SPR. The results are representative of three independent experiments, and expressed as mean ± s.e.m. with each dot representing the data of one sample. Statistical analysis was generated using an unpaired, two-tailed Student’s t test. **P < 0.01