Table 1.
Case no. | Culprit drug | Symptom | CDR3 clonotypes | TRBV | TRBJ | Frequency (%) | Specific CDR3 reads/total reads | Frequency (%) in PBMC of healthy donorsa | |
---|---|---|---|---|---|---|---|---|---|
Case 1 | CBZ | SJS/TEN | 1 | ASSLSDTIY | 12–4 | 1–3 | 24.45 | 182480/746406 | 0 |
2 | ASSLGGAPY | 12–4 | 2–7 | 8.41 | 62776/746406 | 0 | |||
3 | ASSYNPGTGTEEYEQY | 6–1 | 2–7 | 6.50 | 48516/746406 | 0 | |||
4 | ASTHTGELF | 12–4 | 2–2 | 4.60 | 34325/746406 | 0 | |||
5 | ASSLAGELF | 12–4 | 2–2 | 3.73 | 27829/746406 | <0.001 | |||
6 | ASSPRLAGSTDTQY | 27 | 2–3 | 2.11 | 15748/746406 | <0.001 | |||
Case 2 | CBZ | SJS | 1 | ASSLAGELF | 12–4 | 2–2 | 19.39 | 153620/792164 | <0.001 |
2 | ASSLSGYEQY | 27 | 2–7 | 4.01 | 31781/792164 | 0.0026 | |||
3 | ASSSAGEVF | 12–4 | 2–1 | 2.27 | 17990/792164 | 0 | |||
Case 3 | CBZ | SJS | 1 | ASSLAGELF | 12–4 | 2–2 | 6.64 | 5411/81449 | <0.001 |
2 | ATSGPNQETQY | 24–1 | 2–5 | 2.08 | 1697/81449 | 0 | |||
Case 4 | CBZ | TEN | 1 | ASSLAGELF | 12–4 | 2–2 | 42.85 | 31148/72692 | <0.001 |
2 | ASSYRDGYEQY | 6–5 | 2–7 | 2.73 | 1986/72692 | <0.001 | |||
3 | ASSRRAVVGSYNEQF | 7–2 | 2–1 | 2.62 | 1905/72692 | 0 | |||
4 | ATHGTGYLEQY | 25–1 | 2–7 | 2.15 | 1565/72692 | 0 | |||
Case 5 | CBZ | SJS/TEN | 1 | ASSLAGELF | 12–4 | 2–2 | 17.41 | 143248/822649 | <0.001 |
2 | ASSSRLAGGTDTQY | 27 | 2–3 | 12.11 | 99586/822649 | 0.0025 | |||
Case 6 | CBZ | SJS/TEN | 1 | ASSPSDRSSYEQY | 18 | 2–7 | 4.69 | 57694/1229012 | 0 |
2 | ASSSLTSSWVEQF | 28 | 2–1 | 3.81 | 46784/1229012 | 0 | |||
3 | ASTSGPNEQF | 12–4 | 2–1 | 2.99 | 36759/1229012 | <0.001 | |||
4 | ASSYSSTDTQY | 6–5 | 2–3 | 2.80 | 34429/1229012 | 0.0029 | |||
5 | ASSQYRYNEQF | 14 | 2–1 | 2.60 | 32006/1229012 | 0 | |||
6 | ASSFAGELF | 12–4 | 2–2 | 2.22 | 27338/1229012 | <0.001 | |||
7 | ASSLAGELF | 12–4 | 2–2 | 1.08 | 13273/1229012 | <0.001 | |||
Case 7 | CBZ | TEN | 1 | ASSWDPTIY | 12–3 | 1–3 | 10.21 | 81449/798017 | 0 |
2 | ASSLAGELF | 12–4 | 2–2 | 4.00 | 31881/798017 | <0.001 | |||
3 | ASIDGSSLNEQF | 27 | 2–1 | 3.57 | 28498/798017 | 0 | |||
4 | ASSLSGYEQY | 27 | 2–7 | 2.90 | 23122/798017 | 0.0026 | |||
5 | ASSYSDTIY | 6–6 | 1–3 | 2.50 | 19948/798017 | 0 |
CDR3 third complementarity-determining region, PBMC peripheral blood mononuclear cell, SJS Stevens–Johnson syndrome, TEN toxic epidermal necrolysis, TRBJ T-cell receptor β joining, TRBV T-cell receptor β variable
aThe mean frequencies of the corresponding CDR3 clonotypes in PBMC of healthy donors (n = 44)