Fig. 3.

Removal of the FQNLF motif and flanking residues prevents the interaction of cytosolic AR with Hsp40 and Hsp70 in cells. a The interaction between FLAG-AR as well as FLAG-AR-del with endogenous Hsp70 in the absence or presence of 1 nM DHT was studied in transfected HEK293T cells by PLA (n = 3). EV stands for empty vector, FLAG-AR/Hsp70 complexes appear as red dots and DAPI (blue) indicates the localization of nuclei. b Analysis by Western blotting of the levels of FLAG-AR and FLAG-AR-del in transfected HEK293T cells in the absence or presence of 1 nM DHT (n = 3). β-actin immunoblot is shown as a loading control. Log2 transformed values were used to assess differences using two-tailed unpaired t-tests and ***p < 0.001. c Assay equivalent to that presented in a to study the interaction with the endogenous Hsp40 (n = 3). The scale bars in a, c represent 10 μm. Source data for the comparison of protein levels shown in b are available as Source Data file