Fig. 7.

JG-294 promotes clearance of AR113Q in mouse skeletal muscle. Three-month-old AR113Q males were injected i.p. with JG-294 (3 mg kg⁻¹), 17-DMAG (10 mg kg⁻¹), or vehicle every other day for two weeks. a Protein lysates from quadriceps were analysed by western blot for AR and GAPDH. b Quantification of AR monomer and AR high molecular weight species by densitometry. Data are mean ± S.E.M. from three mice per group. **p < 0.01 by two-tailed t-test. V, Vehicle; JG, JG-294. c Relative expression of AR mRNA was determined by qPCR. Data are mean ± S.E.M. from three mice per group. d Quadriceps muscle protein lysates were analyzed by agarose gel electrophoresis for resolution of aggregates (AGERA) followed by western blot for AR. e Quadriceps were stained for AR (red) and analyzed by immunofluorescence microscopy. Nuclei were stained by DAPI (blue). Scale bar = 100 μm. At right, Quantification of AR signal within nuclei. Data are mean ± S.E.M. from three independent experiments. ***P < 0.0001 by two-tailed t-test. f Protein lysates from quadriceps were probed by western blot for Hsp70, Erk1/2 and GAPDH. Source data for the quantifications shown in panels b, c, and e are available as Source Data file