Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 8;10:3566. doi: 10.1038/s41467-019-11470-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — Sequence of Fe-S cluster assembly and functional role of FDX2. a Persulfidation of ISCU monitored by the alkylation assay in the NIAUF complex containing either Fe-ISCU or Zn-ISCU without L-cysteine, with L-cysteine and after addition of FDX2. b Persulfidation of the C104S mutant of Fe-ISCU and of apo-ISCU in the NIAUF complex upon addition of L-cysteine. c–e ESI-Q/TOF deconvoluted mass spectra of Fe-ISCU upon incubation of the NIAUF complex without (c) or with (d) L-cysteine and after Fe-S cluster reconstitution (e), analyzed under denaturing conditions (see Supplementary Fig. 4 for raw spectra and Supplementary Table 3 for expected masses). f Fe-S cluster formation monitored at 456 nm upon addition of FDX2 to the NIAUF complex containing persulfidated Fe-ISCU. g Effect of a metal chelator (DTPA) on persulfide reduction by FDX2. h Effect of FDX2 on the persulfide of NFS1 and Fe-ISCU for various amounts of L-cysteine indicated as molar ratios. i Quantifications of the gels in h expressed as the relative percentages of persulfidated (gray bar) and non-persulfidated (white bar) proteins. Error bars represent standard deviation from n = 3 independent experiments, uncropped gels underlying panels a, b, g, h and source data of panel i are provided as Source Data file