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. 2019 Aug 8;10:3566. doi: 10.1038/s41467-019-11470-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Fe-S cluster reconstitution with Zn-ISCU with excess L-cysteine. a Kinetics of Fe-S cluster assembly by Zn-ISCU (100 μM) monitored by CD at 430 nm, performed under catalytic conditions with the ISC components (NFS1-ISD11-ACP, FXN, FDX2, and FDXR), iron (200 μM) and various amounts of L-cysteine as indicated. b CD spectra of Fe-S cluster reconstitution assays by Zn-ISCU as performed in a with 1 mM of L-cysteine. The spectra were normalized to the spectrum obtained with Fe-ISCU. c Kinetics of Fe-S cluster assembly by Zn-ISCU with 10 equivalents of L-cysteine in standard conditions (blue) and in reactions missing FXN (red) or FDX2 (green);