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. 2019 Aug 8;9:11529. doi: 10.1038/s41598-019-47872-4

Figure 2.

Figure 2

PM4.0 exposure increases the activation of the NLRP3-dependent and NF-κB-dependent pathways in the lung tissue of NF-κB-luciferase+/+ transgenic mice. (a) Western blot analysis of the protein expression levels of NLRP3, p-NF-κB, caspase-1 and IL-1β in different groups. (b) Quantification of the protein expression levels by normalization to the internal control, β-actin. (c) Western blot analysis of the protein expression levels of IL-6, TNF-α and IL-4 in the lung tissue of transgenic NF-κB+/+ mice. (d) Quantification of protein expression levels by normalization to the internal control, β-actin. Representative images of protein expression levels assayed by Western blotting. n = 8 per group. Data are expressed as the mean ± SD. *p < 0.05, ***p < 0.001 compared to the control group. #p < 0.05 compared to the 10 mg/kg PM4.0-treated group.