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. 2019 Aug 8;10(8):598. doi: 10.1038/s41419-019-1837-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a, b The percentage of mo-MDSCs in the bone marrow and spleen of tumor-bearing mice was analyzed by flow cytometry. The samples were analyzed on the second, third, and week 4th of tumor-bearing. c The CXCR2 positive cells in bone marrow were analyzed by flow cytometry. The mice were treated as described in a, b. d Absolute number of mo-MDSCs in the blood, bone marrow, and spleen of control mice or mice bearing tumors after 3 weeks. e The number of metastatic foci was detected in the lungs of WT control mice, CXCR2−/− control mice, 2-week WT, 2-week CXCR2−/−, and adoptively transferred mo-MDSCs in two-week CXCR2−/− tumor-bearing mice. All mice were sacrificed at 2 weeks after a tail vein injection of B16F10 cells. The transferred mo-MDSCs were isolated from the blood of WT tumor-bearing mice. f B16F10 cells were subcutaneously inoculated into WT or CXCR2−/− mice, and the size of tumors was measured at the indicated time points. Bars represent the mean ± SD of five independent experiments. A one-way ANOVA with repeated measures followed by a Dunnett’s post hoc test or a two-way ANOVA followed by Holm-Sidak’s post hoc test were used to determine the level of statistical significance (*p < 0.05; **p < 0.01; and ***p < 0.001; ns, not significant)