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. 2019 Aug 2;7:126. doi: 10.3389/fcell.2019.00126

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Copyright © 2019 Liu, Majeed, Grigaitis, Betts, Climer, Starkuviene and Storrie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Rab6 and CATCHR protein depletion effects on Golgi organization as revealed by wild field microscopy (32× objective) and EM. HeLa cells stably transfected with GalNAcT2-GFP as a Golgi marker were used in panels (A–N). RNAi treatments were at a concentration of 100 mM as described in Section “Materials and Methods” and cells were processed for microscopy 4 days post initial transfection. siRNAs directed against (A) scrambled, Control, (B) ZW10, (C) COG3. (D) Rab6, (E) Rab6+ZW10, (F) Rab6+COG3, (G) scrambled, Control, (H) Rab6 (Young), (I) Rab6A, (J) Rab6A’, (K) ZW10, (L) COG3, (M) Rab6+ZW10, (N) Rab6+COG3.