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. 2019 Aug 2;7:126. doi: 10.3389/fcell.2019.00126

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Copyright © 2019 Liu, Majeed, Grigaitis, Betts, Climer, Starkuviene and Storrie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Wide field microscopy (32× objective) revealed suppressive effects of Rab or Kif co-depletion on CATCHR protein-dependent Golgi fragmentation. Reference single siRNA treatments: (A) siControl, (B) siZW10, and (C) siCOG2. (D–L) Tests of the suppressive effects of various Rab siRNAs: (D–I) Rab27A and Rab33B as positive examples of suppression of Golgi fragmentation and (J–L) Rab30 as a negative example of suppression. (M–R) Tests of the suppressive effects of Kif siRNA SMARTPool examples.