Table 2.
Summary of Rabs and Kifs knockdown screening against ZW10 or COG3 knockdown.
| Basal effect on Golgi apparatus | Effect on Golgi fragmentation | ||
|---|---|---|---|
| Rabs | |||
| Fragment Golgi apparatus (2) | Rab1A, Rab2A | Suppress both ZW10 and COG3 KD (6) | Rab6, Rab6A, Rab6A’, Rab22A, Rab27A, Rab39A |
| No effect alone (18) | Rab1B, Rab4A, Rab6, Rab6A, Rab6A’, Rab29, Rab8A, Rab10, Rab11A, Rab14, Rab22A, Rab27A, Rab30, Rab33B, Rab34, Rab38, Rab39A, Rab43, | Suppress ZW10 KD only (3) | Rab29, Rab11A, Rab33B |
| Suppress COG3 KD only (0) | None | ||
| Non-suppressive (9) | Rab1B, Rab4A, Rab8A, Rab10, Rab14, Rab30, Rab34, Rab38, Rab43 | ||
| Kifs | |||
| Fragment Golgi apparatus (10) | Kif4A, Kif10, Kif11, Kif12, Kif14, Kif15, Kif21A, Kif24, Kif26A, Kif26B | Suppress both ZW10 and COG3 KD (2) | Kif25, KifC3 |
| Stretched strands of Golgi apparatus (2) | Kif20A, Kif23 | Suppress ZW10 KD only (10) | Kif2C, Kif3A, Kif4B, Kif5A, Kif6, Kif7, Kif9, Kif14, Kif17, KifC2 |
| Toxic to HeLa cells (1) | Kif18A | Suppress COG3 KD only (0) | None |
| No effect alone (31) | Kif1A, Kif1B, Kif1C, Kif2A, Kif2B, Kif2C, Kif3A, Kif3B, Kif3C, Kif4B, Kif5A, Kif5B, Kif5C, Kif6, Kif7, Kif9, Kif13A, Kif13B, Kif16A, Kif16B, Kif17, Kif18B, Kif19, Kif20B, Kif21B, Kif22, Kif27, KifC1, KifC2, KifC3, Kif25 | Non-suppressive (23) | Kif1B, Kif1C, Kif2A, Kif2B, Kif3B, Kif5B, Kif5C, Kif10, Kif11, Kif12, Kif13B, Kif16A, Kif16B, Kif18A, Kif18B, Kif19, Kif20B, Kif21A, Kif21B, Kif24, Kif26A, Kif26B, Kif27 |
Rabs highlighted in red were further tested by electron microscopy. Kifs highlighted in red were further tested by electron microscopy.