Fig. 5.

Systematic analysis of ecYeast8 and proYeast8^DB underlies the potential relations between gene mutations and growth phenotype. a Pipeline to conduct the integrative analysis of ecYeast8 and proYeast8^DB. b Distribution of relative growth rate with glycerol as carbon substrate in complex medium. c Three groups of strains with high, medium and low relative growth rates used for hotspot analysis. d Heatmap of hotspot zones for three groups of strains and heatmap of flux control coefficients obtained from ecYeast8 with growth as the objective function. e Mapping of all YJL052W mutations from strains with high growth rate onto the protein 3D structure. f Position of enzyme encoded by YJL052W in the glycerol utilisation pathway. g Classification of the remaining strains based on mutations in hotspot zones and non-hotspot zones of YJL052W. The statistical analysis is based on Wilcoxon rank sum test