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. 2019 Aug 8;9:11541. doi: 10.1038/s41598-019-47573-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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YM155 suppresses Mcl-1 protein levels, but not mRNA levels and such protein may occur partly through a proteasomal-independent mechanism. (A,B) PC-3, DU-145, and RCC4 cells were treated with the indicated concentrations of YM155 for 8 and 24 h, and Western blotted for Mcl-1 and β-Actin. (C,D) Effect of YM155 on Mcl-1 mRNA expression in PC-3 was assessed by semi-quantitative RT-PCR (C) and quantitative real-time PCR (D). (E) PC-3 cells were pretreated for 30 min with 5 or 10 µM MG132, followed by treatment with 100 nM YM155 or vehicle for 4 h, after which cells were subjected to Western blotting for expression of Mcl-1 and β-Actin. (F) The combined effect of MG132 and CHX (30 min) on the YM155-induced suppression of Mcl-1 in PC-3 cells after 4 h, assessed by Western blot. Unless indicated on the figure, each group of blots represents data obtained from a single gel and experiment. Results shown are representative of 2 to 3 independent experiments.