Fig. 1.
Construction of recombinant baculoviruses expressing thogotovirus GPs. A The schematics of recombinant viruses. The left panel shows the schematic representation of recombinant AcMNPV bacmids carrying the reporter gene egfp and the thogotovirus GPs fused with HA tag at C-terminal. The right panel shows the baculoviruses presenting thogotovirus GPs in a gp64-null bacmid, where the gp64 gene was substituted by chloramphenicol resistance gene (Chlr) and egfp. B Transfection and infection assay of recombinant viruses. Sf9 cells were transfected by the recombinant bacmid DNA of Ac-WT, Ac-ThGPHA, Ac-DhGPHA, AcΔgp64-ThGPHA and AcΔgp64-DhGPHA. The cells were imaged at 96 h post transfection and the supernatants were collected to infect Sf9 cells and imaged at 96 h p.i. Bar, 200 μm.