Fig. 2.
The incorporation of thogotovirus GPs in the AcMNPV virions and its impact on virus infection. A The subcellular localization of thogotovirus GPs in infected cells. Sf9 cells were infected by Ac-WT, Ac-ThGPHA or Ac-DhGPHA and fixed at 48 h p.i. The thogotovirus GPs were detected by anti-HA antibody and Alexa flour 647 goat anti-mouse antibody (Cy5). The cell nuclei were stained by Hoechst33258 (blue). FITC channel indicates the EGFP expression in the virus infected cells. Bar, 5 μm. B Detection of the incorporation of thogotovirus GPs in the virions. The virions of recombinant viruses were purified and analyzed by Western blot assay using anti-HA antibody. The major viral capsid protein VP39 was detected by anti-VP39 antibody as control. C One-step growth curve analysis of recombinant viruses. Sf9 cells were infected by Ac-WT, Ac-ThGPHA and Ac-DhGPHA at an MOI of 5 TCID50 units/cell. The supernatants were collected at indicated time points and the virus titer was determined by endpoint dilution methods. The results are mean ± SD (n = 3 experimental replicates).