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. 2019 Apr 24;34(4):386–396. doi: 10.1007/s12250-019-00111-6

Fig. 2.

Fig. 2

Design of an artificial miR-H6-5p sponge. A Schematic representation and sequence of the designed miR-H6-5p sponge to miR-H6-5p. Complementary sequences are indicated. The nucleotides in green and light brown indicate the mismatch region and nucleotides in red indicate linker bridges between the 10 repeats of the sponge. The miR-H6-5p sponge was subcloned into the 3′ UTR of the Renilla gene of psiCHECK™-2 Luciferase vector to make the luc-H6-5p sponge plasmid. B Validation of biological function of the miR-H6-5p sponge with plasmid psiCHECK™-2. Co-transfection of luc-H6-5p sponge plasmid with either mock, or 20 or 40 nmol/L of non-target (NT) miRNA mimic or with miR-H6-5p mimic for 24 h. Decreases in luciferase activity correlate with the ability of the miR-H6-5p sponge to sequester miR-H6-5p. The two independent experiments performed in triplicate are shown. *P < 0.05, one-way ANOVA.