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. 2019 Apr 24;34(4):386–396. doi: 10.1007/s12250-019-00111-6

Fig. 3.

Fig. 3

Accumulation of viral miRNAs H6-5p, H6-3p, H1-5p, and H1-3p in HEp-2 cells transfected with the plasmid encoding miR-H6-5p sponge. A Schematic representation of plasmids designed to express EGFP-fused miR-H6-5p sponge (P1065), a scrambled sequence (P1066), and no-sponge (P1000) under the Egr-1 promoter. B Expression levels of miRNAs in HEp-2 cells in 12-well plates after transfection with 1 μg of P1065, P1066, or P1000 for 24 h normalized to 18s rRNA. The two independent experiments performed in triplicate are shown. *P < 0.05; **P < 0.01, one-way ANOVA comparing the P1065 group to the other groups at the same time point.