Fig. 4.
Decreased viral yield and protein expression following infection with recombinant HSV (R1065)-expressing miR-H6-5p sponge. A Schematic representation of the cassette of the Egr-1 promoter, EGFP-fused miR-H6-5p sponge inserted into the wild-type viral genome between the UL3 and UL4 genes. B Accumulation of viral proteins in cells infected with wild-type HSV-1(F) and the recombinant R1065. The result is representative of two independent experiments, and the numbers under the blots indicate the relative expression calculated by ImageJ software. C Virus yields of R1065. The two independent experiments were performed in triplicate. Student’s t test was performed to compare groups at the same time point (*P < 0.05). D and E Expression levels of miRNAs in HEp-2 cells exposed to 10 PFU of R1065 normalized to 18s rRNA. The two independent experiments (each experiment was performed in triplicate) are shown. Student’s t-test was performed to compare groups at the same time point (**P < 0.01).