Fig. 5.
Viral gene expression during virus reactivation from R1065 latently infected ganglia. Trigeminal ganglia were excised from six mice 30 days after corneal inoculation of 105 PFU of HSV-1(F) or R1065, and incubated in 199 V medium containing anti-NGF antibody for 0 or 24 h. The geometric mean of amounts of viral mRNAs encoding ICP27, ICP8, VP16, and LAT per groups of six ganglia selected at random are shown. The two independent experiments of qPCR using cDNAs as templates were performed in triplicate (**P < 0.01).