Table 5.

Primers and annealing temperatures for species-specific discrimination of DNA in soil samples.

Species	Forward primer (5′–>3′)	Reverse primer (5′–>3′)	Annealing temperature	Amplification in region
Mustard	TTTCTTTGCTGATTCTGTGCCTG	CGAAGTACTGGCTGGGAACTTAA	65 °C	ITS1
Phacelia	GGTTGTTATCTCAACTCGCGTG	GGTCTATTCAGTCCCGGCAG	64 °C	ITS2
Oat	TAAACACGCTCCCAACCCCTTA	TCGGAGACACTGCGGTAAGTATAG	66 °C	ITS2
Clover	TGAATTAGTTTCAACACATAGGGTTGGTTC	GAGCAAATTTTAAATTCCTTGACGCATTCAG	63 °C	ITS1
Spathiphyllum	CTCTGTCTGCCTGCCTATTTGTT	GTCATTCAGACTTAAACTTGCGACG	62 °C	ITS1