Fig. 3.
Characterization of complemented-defective OHFV-ΔNS1 virus. A Growth kinetics of OHFV-ΔNS1 virus in BHK21NS1 cells. B Genetic stability of OHFV-ΔNS1 virus. Total RNA was extracted from BHK21NS1 cells infected with P1–P5 viruses. RT-PCR was performed with primer pairs spanning E and NS2A. As a control, WT represents the products obtained by PCR using OHF-IC as a template. C Stability and biosafety of defective OHFV-ΔNS1 virus. BHK21NS1 and BHK21 cells were infected with equal volumes of P1, P3, and P5 OHFV-ΔNS1 virus, followed by IFA analysis.