Fig. 6.
Antiviral activity of NITD008 on OHFV-ΔNS1-Gluc virus in BHK21NS1 cells. A Antiviral activity of NITD008 on OHFV-ΔNS1-Gluc virus replication. BHK21NS1 cells were infected with OHFV-ΔNS1-Gluc virus and incubated with different concentrations of NITD008. After 48 h, cells were lysed and Gluc activity was examined. Three independent experiments were performed in duplicate, with error bars indicating standard deviation. B Optimization of HTS assay using OHFV-ΔNS1-Gluc viruses. Different densities of BHK21NS1 cells were seeded into a 96-well plate. Cells were infected with OHFV-ΔNS1-Gluc virus and incubated with a medium containing 3 μmol/L NITD008. At 24 hpi, the cells were lysed and assayed for luciferase activity. The Z′ value was calculated to evaluate the effectiveness of the HTS assay.