Table 1.
Primer and probe combinations used in this study.
| Primer/Probe | Sequence (5′ –> 3′) | Reference | Amplicon size in combination with Ams189R |
|---|---|---|---|
| Primers | |||
| Ams189R† | GGG TAT TTG CGC TAA TTT TAT TCG | Pirc et al., 2009 | |
| Ams116F | TCC CAC ATA CTG TGA ATC ATC CA | Pirc et al., 2009 | 74 bp |
| Ams03KbF | ATA TCC TTG TTG ATA AAC AGG TGC G | This study | 325 bp |
| Ams06KbF† | AAT TGG TTC CGC TAT AAC TTG CAG | This study | 627 bp |
| Ams09KbF | TAA TAG ACT GAG CTG AAA GTA TCG TGT | This study | 966 bp |
| Probe | |||
| Ams141T | FAM-CCA GAA TCT GGC CCG CGT ATA CCG-TAMRA | Pirc et al., 2009 | |
†Primer combination used in the final optimized protocol for dPCR and v-dPCR.