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. 2019 Aug 8;9:11506. doi: 10.1038/s41598-019-47743-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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H&E staining of mice colon. (a,b) Normal colon at 10× and 60× magnifications; (c,d) Initiation of colon adenocarcinoma after treatment with DMH for a 7 weeks (at 10× and 60× magnifications respectively); (e,f) Development of colon adenocarcinoma after completion of treatment regimen with DMH (12 weeks) (at 10× and 60× magnification respectively); (g,h) Improvement of cancerous region after completion of treatment of colorectal cancer mice treated with 2c-NP (9 weeks) (at 10× and 60× magnifications respectively). (I,j) Normal colon after treatment with 2c-NP (at 10× and 60× magnifications respectively). Scale bar used in 10× and 60× magnification panels represent 200 µm and 20 µm respectively.