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. 2019 Aug 8;9:11506. doi: 10.1038/s41598-019-47743-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Acridine Orange/Ethidium Bromide (AO/EB) dual staining. Confocal microscopy images of HT-29 cells after treating with 2c-NP (11.8 μM; 0–48 h) followed by staining with acridine orange (4 µg/ml) and ethidium bromide (4 µg/ml) as observed under Olympus Fluoview 10i confocal microscope at 100X magnification. Scale bar represents 10 µm.