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. 2019 Aug 8;9:11507. doi: 10.1038/s41598-019-47977-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Kinetics of oxidation of total and exogenous metabolic substrates after gavage of a labelled liquid mixed meal, in mice fed a HFD for nine weeks (second mouse study). (a) Instantaneous total (n = 47) and exogenous (n = 23) glucose oxidation after administration of the ¹³C glucose liquid mixed meal until the end of the measurements. (b) Data from panel (a), emphasizing the early 2 h postprandial period. (c) Instantaneous total fat oxidation (n = 47) and exogenous palmitate oxidation (n = 24) after administration of the ¹³C palmitate liquid mixed meal. (d) Data from panel (c), emphasizing the early 2 h postprandial period. (e) Cumulative exogenous glucose (n = 23) and palmitate oxidation (n = 24) after administration of the labelled liquid mixed meals until the end of the measurements. (f) Data from panel (e), emphasizing the early 2 h postprandial period. Exogenous substrate oxidation data is calculated from conventional InCa data (VCO₂) and ¹³CO₂ and ¹²CO₂ data. Shaded areas represent the dark phase. Data is presented as mean ± SD. InCa, indirect calorimetry; PW, postnatal week.