FIGURE 2.

Mapping the sequence determinants of –10 motifs in E. chaffeensis dnaK gene promoter. The β-galactosidase expression driven by E. chaffeensis promoter constructs containing point mutations at each of the six nucleotide positions of the –10 motifs of dnaK were measured in CAG57101 expressing E. chaffeensis σ³². The experiment included the wildtype promoter control (WT). Each mutation is identified with a change of the nucleotide at each position to the modified nucleotide. β-galactosidase expression was presented relative to WT control. All values are averages of at least three independent experiments; error bars indicated one standard deviation. ^*p-value < 0.05.