FIGURE 5.

Substitutions at six amino acids located in regions 2.3, 2.4, and 3.0 of E. chaffeensis σ³² impacting the promoter activity of the wild-type E. chaffeensis dnaK. Mutations at six residues (Y102, W106, A109, F110, Q128, and F134) of E. chaffeensis σ³² were assessed with the wild-type dnaK promoter. β-galactosidase expression was measured for the mutant proteins relative to the wild-type (WT) E. chaffeensis σ³² in CAG57101. All values are averages of at least three independent experiments; error bars indicated one standard deviation. ^*p-value < 0.05.