Figure 2.

Allele‐specific regulation of the chemokine CXCL12 by MIR938. (a) Sequence alignment between the MIR938 rs12416605 C and T alleles and the CXCL12 mRNA showing the PITA predicted target site and ΔΔG PITA scores for both alleles on this site. (b) Results of the luciferase‐reporter assay testing the interaction between MIR938 rs12416605 C and T alleles and the 3′UTR of the CXCL12 in HeLa cells. Ratios of the Firefly and Renilla luciferase luminescence are presented after normalization to the empty plasmid pGL4.13. Each experiment sswas done in triplicate and six independent experiments were performed. Data reported here are the means ± SEM of all experiments performed. A significant reduction in the luciferase activity between both the alleles and between the C allele and the control miRNA was found (*, p < 0.01, Student's t‐test)