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. 2019 Aug 8;9:11519. doi: 10.1038/s41598-019-47993-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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KUS121 has no effect on the viability of oligodendrocytes under ischemia. (a) Western blot analyses of rat primary cortical neuron and oligodendrocyte lysates for VCP. The ratio of VCP to β-actin expression levels was calculated (n = 4). (b) Cell viability measured by the WST (water-soluble tetrazolium salt) assay. Rat primary oligodendrocytes were exposed to vehicle (dimethyl sulfoxide, DMSO) or KUS121 under control conditions or under OGD for 12 h (n = 4). (c) Measurement of ATP concentration in primary cortical oligodendrocytes exposed to vehicle (DMSO) or 100 μM KUS121 under control or OGD conditions (n = 5).