Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 8;10:3585. doi: 10.1038/s41467-019-11584-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — CIN cancer cells present centriole disengagement. a Representative images of prometaphase DLD1, HCT116, HT29, H747 Cal51, HCC70 and HCC1187 cells stained with antibodies against centrin (green), γ-tubulin (red) and DAPI (blue); scale bars = 10 μm. b Percentages of prometaphase cells with multipolar spindle with either premature centriole disengagement (grey) or extra centrosomes (white) in indicated cell lines; N = 93 prometaphase cells for DLD1, 92 for HCT116, 81 for HT29, 91 for H747, 203 for Cal51, 323 for HCC1187 and 359 for HCC70; p < 0.0001 in a chi-square test (DLD1 vs. HT29 p = 0.001, HCT116 vs. HT29 p = 0.0048, H7474 vs. HT29 p = 0.0011, Cal51 vs HCC1187 p = 0.0214 and Cal51 vs HCC70 p = 0.0066). c Mean percentage of prometaphase HT29, HCC1187 and HCC70 cells with premature centriole disengagement with or without a 20 μM nucleosides supplement; N = 3 examining NT/HT29 = 138, nucleosides/HT29 = 125; NT/HCC1187 = 323, nucleosides/HCC1187 = 350, NT/HCC70 = 359 and nucleosides/HCC70 = 350 prometaphase cells; p < 0.0001 in two-way Anova test. (HT29 p = 0.002, HCC1187 p = 0.0158 and HCC70 p = 0.0065). d–f Quantification of the spindle intensity over time after a nocodazole pulse, as shown in Fig. 2d, in metaphase HT29 (d), HCC70 (e) or HCC1187 cells (f) supplemented with or without nucleosides; N = 33 for HT29/NT and 23 for HT29/nucleosides, 74 for HCC70/NT and 55 for HCC70/nucleosides, 74 for HCC1187/NT and 65 for HCC1187/nucleosides. *p < 0.05; **p < 0.01; ***p < 0.001 in a repeated measurement Anova test. Error bars indicate sem. Source data are provided as a Source Data file