Figure 4.

The effect of mouse double minute 4 (MDM4) on cell growth in renal cell cancer (RCC). (a, b, c) RT‐qPCR and western blot analysis of MDM4 expression in RCC cells. (d) Kaplan–Meier analysis of overall survival in 30 RCC patients with low median (n = 15) and high median (n = 15) expression levels of MDM4. (e) CCK‐8 assays indicated that the effects of MDM4 on growth of RCC cell lines. Results were expressed as ±$\stackrel{-}{\mathrm{x}}$s of three independent experiments, with at least three replicates in each independent experiment, *p < 0.05 versus NC. (f) Western blot analysis of p53 expression after miR‐33a mimics and ‐inhibitor transfection in RCC cells