No difference in Nav1.6 puncta in soma, dendrites, or axons between WT and ARF4+/−/ARF5−/− PCs. A, B, Sagittal sections of postnatal week 8 (P8w) WT (A) and ARF4+/−/ARF5−/− (B) mouse cerebella were immunolabeled with an anti-Nav1.6 antibody (green) and an anti-calbindin (magenta) antibody. Scale bars, 20 μm. C, D, Densities of Nav1.6-positive puncta in primary and secondary dendrites (C) and soma (D) of WT (white; dendrite, n = 40; soma, n = 48) and ARF4+/−/ARF5−/− PCs (black; dendrite, n = 40; soma, n = 61). E, F, Sagittal sections of P8w WT (E) and ARF4+/−/ARF5−/− (F) cerebellar white matter were immunolabeled with an anti-Nav1.6 antibody (green) and an anti-calbindin (magenta) antibody. Enlarged images from rectangular white regions are shown in insets. Scale bars, 10 μm. G, Densities of Nav1.6-positive puncta in cerebellar white matter of WT (white; n = 34) and ARF4+/−/ARF5−/− PCs (black; n = 21).