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. 2019 Apr 2;28(16):2635–2647. doi: 10.1093/hmg/ddz068

Figure 1.

Figure 1

(A) demonstrates the Chkb transgene construct with a functional copy of Chkb cDNA-inserted downstream of a complete exon 1 and partial exon 2 structure followed by a SV40Poly A site downstream of the Chkb cDNA. (B) shows a WT (+/+), Tg-rmd and rmd (rmd/rmd) mouse from top to bottom with the Tg-rmd mouse being physically comparable to the WT. Chkb gene expression in muscle is 18-fold higher in Tg-rmd and 14-fold higher in Tg-WT mice as seen from real-time PCR analysis, whereas expression in rmd mice was not detectable and that in +/rmd mice was 0.21 times higher than in rmd mice (C). Tg-rmd, +/rmd (unaffected littermates) and Tg+/rmd mice do not show significant differences in their body weights when measured starting at 3 weeks of age (post-wean; D). The Tg-rmd and Tg-WT mice are physically indistinguishable from each other (E). N = 4 mice/sex/genotype.