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. 2019 Aug 2;9:630. doi: 10.3389/fonc.2019.00630

Figure 3.

Figure 3

Effect of digitoxin on HSPB1 and RBFOX2 mRNA expression in PC3 cells. (A) Effect of digitoxin (25 nM) on HSPB1 mRNA expression. Neither digitoxin (25 NM) nor TNFa (10 ng/ml) have significant effects on HSPB1 mRNA expression. However, both TNFa and digitoxin together significantly reduce HSPB1 expression (P = 0.0239; N = 3). Three day exposure; Data are from RNA-seq experiments. TPM are transcripts per million. (B) Effect of digitoxin (25 nM) on HSPB1/RBFOX2 mRNA ratio. Exposure of cells to digitoxin alone, or TNFα alone, do not exert a significant effect in the ratio. However, in the presence of both TNFα and digitoxin, the ratio is significantly reduced by ca. 41% (P = 0.0179; N = 3). Three day exposure, Data are from RNA-seq experiments. TPM are transcripts per million.