Table 3.

Digitoxin effects on microRNA content in primary tumors.

MicroRNA	p-value	DDCt [Test, A–Control, B]	Fold-change (corrected to absolute values)
A
miR-346	0.034	+5.05	−33.1
miR-98/let7a-5p	0.019	−3.18	+9.06
miR-448-3p	0.026	−3.93	+15.24
miR-211-5p/miR-204-5p	0.040	−4.59	+24.08
B

Panel A, DDCt (delta delta Ct) takes into account both the sample and a control. A negative or positive DDCt value corresponds to an increase or decrease, respectively, in fold change. A (+ digitoxin), n = 10; B (control), n = 11. Panel B, IPA analysis comparing predicted interactions of microRNAs with digitoxin-dependent mRNAs. Color Code: red (increased expression); green (decreased expression).