Figure 5.

Effect of miR-181a-2-3p on multiple intracellular signaling pathways. BEAS-2B cells were pretreated with or without inflammatory signal downstream inhibitors (10 µM) for 1 h. Then, cells were transfected with/without miRNA 181a-2-3p inhibitor and then stimulated with 10 µM Cd for 24 h. 2-APB (A), U73122 (B), DPI (C), and TAK202 (D) were analyzed by immunoblotting, and intensities of bands were quantified and normalized with internal control β-actin by Image J software. All data shown are representative of at least three independent experiments. *, P<0.05; **, P<0.01 were considered significant. NS, not significant; Cd, cadmium; DPI, diphenyleneiodonium; 181m, 181 mimics; 181i, 181 inhibitor.