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. 2019 Aug 2;10:1839. doi: 10.3389/fimmu.2019.01839

Figure 7.

Figure 7

Injection of rOVA-FLIPr increases the frequency and maturation of CD8+ dendritic cells in lymph nodes. Groups of C57BL/6 mice were injected with rOVA, rOVA-FLIPr (100 μg) in the left hind foot pad. Mice injected with PBS were used as controls. Right (injection:–) and left (injection: +) inguinal lymph nodes were harvested 24–48 or 72–96 h after injection. (A) The frequencies of CD8+ DCs, CD8− DCs, and pDC were analyzed by flow cytometry. (B) The expression of CD40 and MHC II on the surface of CD8+ DCs, CD8− DCs, and pDC were analyzed by flow cytometry. The relative mean fluorescence intensity (MFI) of each mouse was calculated by the formula: relative MFI = (MFI of cells obtained from left (injection: +) inguinal lymph node)/(MFI of cells obtained from right (injection: –) inguinal lymph node). The results were pooled from 2 to 3 independent experiments for each time points. The statistical significance was determined by a paired t-test. *p < 0.05, **p < 0.01, and ***p < 0.0001.